Children's Mercy Hospitals and Clinics
Name
Vitamin D, 25-Hydroxy

CMH Lab Section
Toxicology/TDM

Specimen Requirements
2 mL blood in plain, red-top tube, no gel (preferred). Red gel is acceptable.

Availability
Routine

Turn Around Time
3-7 days

Processing Instructions
Spin and refrigerate serum.

Methodology
Tandom Mass Spectrometry

Ranges
Vitamin D 25OH, total (D2 and D3)  30-100 ng/mL
25-OH vitamin D2 not established
25-OH vitamin D3 not established
Below range: Reportable as <5

Total 25-Hydroxyvitamin D (D2 +D3) levels between 15-29 ng/mL suggest insufficiency, while levels <15 ng/mL suggest deficiency.

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