Children's Mercy Hospitals and Clinics
Name
Flow Cell Viability

CMH Lab Section
Flow Cytometry

Specimen Requirements
3 mL of peripheral blood, peripheral blood progenitor cell (Stem Cell or PC) harvested or thawed products, bone marrow in heparinized syringe or lavender top (EDTA) tube or yellow top (ACD) tube. Tumor aspirates or cell suspensions in RPMI media.

Availability
Routine

Turn Around Time
Prefer specimens are submitted Monday-Friday before 1400. Testing is completed within 2 hrs of receiving.

Special Instructions
No gel tubes

Methodology
The percent of cells that do not take up Propidium Iodide (viable cells) is measured on a flow cytometer.

Ranges

>70% viability required for most procedures

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