Children's Mercy Hospitals and Clinics
Name
Flow Stem Cell Analysis - CD34, Viability Assessment

Synonyms
Progenitor Cell Enumeration - CD34, viability assessment

CMH Lab Section
Flow Cytometry

Specimen Requirements
3 mL blood in EDTA lavender or
1 mL harvested product or thawed peripheral blood stem cell in EDTA lavender or plain red tube or
1 mL harvested or thawed cord blood in EDTA lavender or plain red tube

Availability
Routine

Turn Around Time
2-4 hours

Special Instructions
Enumeration of CD34 positive staining cells in a sample (of total nucleated cells). Viability assessment of specimen also done.

Methodology
Antibody staining for cell surface markers. Analyzed on a flow cytometer. Plus Propidium iodide dye exclusion to assess viability.

Ranges
Not Applicable.

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