Children's Mercy Hospitals and Clinics
Name
Osmotic Fragility

CMH Lab Section
Hematology

Specimen Requirements
3 mL in a sodium heparin (green top no gel)
Samples are only accepted Sunday through Thursday

Availability
Routine

Turn Around Time
2 days

Special Instructions
Transport at room temperature
Samples are only accepted Sunday through Thursday and must be received the day of collection

Methodology
Dilutions in varying concentration of NaCl Solutions

Ranges

Normal RBC osmotic fragility after incubation at 37 degrees

Normal Saline

% Hemolysis

65%

 0 - 15

60%

 0 - 46

55%

 16 - 83

50%

 

 63 - 109

45%

 83 - 112





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