Children's Mercy Hospitals and Clinics
Name
Herpes Simplex PCR - CSF

Synonyms
HSV PCR - CSF

CMH Lab Section
Molecular Infectious Diseases

Clinical Description
Herpes simplex viruses 1 and 2 (HSV-1 and HSV-2) are two species of the herpes virus family, Herpesviridae, which cause variety of illnesses, including mucocutaneous infections, infections of the CNS, and occasionally infections of the visceral organs. HSV is the most common cause of acute sporadic focal encephalitis in Western countries. The incidence of HSV encephalitis is estimated to be 1-4 cases/106 people/year, and nearly 30% of these occur in patients aged < 20 years. Infections in children can include neonatal disease, mucocutaneous infections during childhood and adolescence, and serious disease in individuals who are immunocompromised. Neonatal HSV infection is a disease with high morbidity and mortality rates. Early recognition and treatment of herpes simplex virus infection particularly in CNS are important to reduce the mortality and incidence of serious neurological sequelae in surviving children. Historically brain biopsy with histological staining was the most relia

Specimen Requirements
0.5 mL CSF

Availability
Routine

Turn Around Time
1-2 days

Methodology
The HSV-1/2 typing real-time PCR assay utilizes simultaneous nucleic acid amplification by PCR and detection using MultiCode®-RTx technology (EraGen Biosciences, Madison, WI) for Herpes simplex virus 1 and 2.

Ranges
not detected

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