Children's Mercy Hospitals and Clinics
Name
Flow Granulocyte Oxidative Burst Immune Deficiency Panel

Synonyms
Flow NBT

CMH Lab Section
Flow Cytometry

Specimen Requirements
3 mL of blood in EDTA lavender

Availability
Routine

Turn Around Time
48 hours

Special Instructions
Specimens must be <24 hours old when received in the Flow Cytometry Lab

Friday: Specimens must bedrawn and received in the Flow Cytometry Lab before 1400

Do NOT collect after 1400 Fridays or on weekends

Processing Instructions
Room temperature, do not refrigerate

Methodology
Granulocytes are stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Measurements of unstimulated and stimulated granulocytes are compared.

Ranges
Testing measures granulocyte oxidase activity which is useful in diagnosis of Chronic Granulomatous Disease (CGD).

Interpreted by Pathologist.

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