Children's Mercy Hospitals and Clinics
Name
Flow Granulocyte Adhesion Molecules Immune Deficiency Panel

Synonyms
CD11b (MAC-1) & CD18 Granulocyte Adhesion Molecules

CMH Lab Section
Flow Cytometry

Specimen Requirements
3 mL of blood in EDTA lavendar tube

Availability
Routine

Turn Around Time
48 hours

Special Instructions
Specimens must be <24 hours old when recieved in the Flow Cytometry Lab

Friday: Specimens must be drawn and recieved in the Flow Cytometry Lab before 1400.

Do NOT collect after 1400 Fridays or on weekends

Processing Instructions
Room temperature, do not refrigerate

Methodology
Granulocytes are stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Measurements of unstimulated and stimulated granulocytes are compared.

Ranges
Testing measures the upregulation of CD11b & CD18 adhesion molecules which is useful in diagnosis of Leukocyte Adhesion Deficiency (LAD) or Lazy Leukocyte Syndrome.

Test interpreted by Pathologist.

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