Children's Mercy Hospitals and Clinics
Name
Flow Extended Immune Deficiency Panel

CMH Lab Section
Flow Cytometry

Specimen Requirements
M-F: 3 mL blood in EDTA tube
After 1400 or on weekends, yellow-top (ACD) tube is only acceptable specimen

Availability
Routine

Turn Around Time
24 hrs

Special Instructions
CBC with differential request must accompany Flow Cytometry test requests in order to report results.
Specimens can not be refrigerated.

Methodology
Antibody staining for cell surface markers analyzed on a flow cytometer

Ranges
includes CD2, CD3, CD4, CD8, CD14, CD16,56, CD19, CD25+/CD3+, CD45, HLA-DR+/CD3, CD16,56+/CD3+

Cell Subset

0 Months

1 Month

6 Months

2 Years

4 Years

8 Years

12 Years

21 Years

CD4 %

39 – 63

39 - 63

30 – 55

34 – 52

32 – 52

30 - 55

30 - 55

30 - 59

CD4 Absolute  per cumm

1200 – 4200

1500 – 5100

1900 – 3600

900 – 2900

500 - 2100

450 - 1600

500 - 1700

300 - 1900

CD8%

14 – 35

14 – 35

14 – 35

16 – 35

14 – 35

14 – 35

14 - 35

14 - 38

CD8 Absolute per cumm

400 – 2100

650 – 2500

550 – 2300

600 – 1900

400 - 1100

300 – 1000

250 - 1000

200 - 1000

CD3%

59 – 84

59 – 84

53 – 81

58 – 80

55 – 82

55 – 82

58 - 82

58 - 85

CD3 Absolute per cumm

1600 – 5500

2000 – 6500

1500 – 5600

1600 – 4300

1100 – 3400

900 – 2900

900 - 2600

700 - 2800

CD2 %

55 – 90

55 – 90

55 – 90

55 – 85

55 – 85

55 – 85

60 - 85

60 - 85

CD2 Absolute per cumm

2500 – 5500

3500 – 6500

3000 - 5600

2000 – 4500

1200 – 4000

1100 – 3800

1100 -3200

1000 -3000

CD19 %

11 – 26

11 – 26

11 – 26

9 – 22

6 – 22

6 – 22

6 - 22

6 - 22

CD19 Absolute

350 – 600

500 – 1800

400 – 1800

200 – 1000

150 – 800

100 – 700

75 - 600

50 - 550

CD16,56 Absolute per cumm

80 - 340

80 - 340

80 - 340

80 - 662

80 - 662

80 - 662

80 - 662

80 - 662

CD16,56+/CD3+

Not Established

Not Established

Not Established

Not Established

Not Established

Not Established

Not Established

Not Established

CD25

Not Established

Not Established

Not Established

Not Established

Not Established

Not Established

Not Established

Not Established

Absolute Lymphocytes per cumm

2000 – 6000

2900 – 8300

2200 – 8300

2400 – 5900

1600 – 5000

1400 – 4000

1300 - 3800

1200 - 3600

HLD-DR

Not Established

Not Established

Not Established

Not Established

Not Established

Not Established

Not Established

Not Established



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