Children's Mercy Kansas City
Name
Flow Extended Immune Deficiency Panel

CMH Lab Section
Flow Cytometry

Specimen Requirements
Monday-Friday: 3 mL blood in EDTA tube
Friday after 1400 - Monday 0600: yellow-top (ACD) tube

Availability
Routine

Turn Around Time
24 hrs

Special Instructions
CBC with differential request must accompany Flow Cytometry test requests in order to report results

Processing Instructions
Specimens can not be refrigerated

Methodology
Antibody staining for cell surface markers analyzed on a flow cytometer

Ranges
Includes: CD2; CD3; CD4; CD8; CD19; CD16,56; CD25+/CD3+, HLA-DR+/CD3, CD16,56+/CD3+

Cell subset

Unit of Measure

Birth to 1-month

1-month to 6-months

6-months to 1-year

2-years to 4-years

4-years to 8-years

8-years to 12-years

12-years to 21-years

Over 21-years

CD2

Percent

55-90

55-90

55-90

55-90

55-85

55-85

60-85

60-85

CD2

Absolute/mm3

2500-5500

3500-6500

3000-5600

2000-4500

1200-4000

1100-3800

1100-3200

1000-3000

CD3

Percent

59-84

59-84

53-81

58-80

55-82

55-82

58-82

58-85

CD3

Absolute/mm3

1600-5500

2000-6500

1500-5600

1600-4300

1100-3400

900-2900

900-2600

700-2800

CD4

Percent

39-63

39-63

30-55

34-52

32-52

30-55

30-55

30-59

CD4

Absolute/mm3

1200-4200

1500-5100

1900-3600

900-2900

500-2100

450-1600

500-1700

300-1900

CD8

Percent

14-35

14-35

14-35

16-35

14-35

14-35

14-35

14-38

CD8

Absolute/mm3

400-2100

650-2500

550-2300

600-1900

400-1100

300-1000

250-1000

200-1000

CD19

Percent

11-26

11-26

11-26

9-22

6-22

6-22

6-22

6-22

CD19

Absolute/mm3

350-600

500-1800

400-1800

200-1000

150-800

100-700

75-600

50-550

CD16,56

Absolute/mm3

80-340

80-340

80-340

80-662

80-662

80-662

80-662

80-662


No established ranges for: CD25+/CD3+; HLA-DR+/CD3; CD16,56+/CD3+

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