Children's Mercy Hospitals and Clinics
Name
Flow DNA Ploidy and Cell Cycle Analysis

CMH Lab Section
Flow Cytometry

Specimen Requirements
Bone marrow or peripheral blood in lavender (EDTA) or green top (Na Heparin) tube. No gel tubes.
Cell suspensions (body fluids or tumor aspirates) in RPMI media.

Availability
Routine

Turn Around Time
3 days

Methodology
Propidium iodide dye binding to DNA after cell membrane permeabilization. Analyzed on a flow cytometer

Ranges
Interpreted by Pathologist.

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