Chlamydia and Gonorrhea PCR
CMH Lab Section
Molecular Infectious Diseases
Source: Urine or swab
Please read Special Instructions before collection.
Collection containers are available from the Virology Lab (816)234-3060.
Specimens must arrive in the Lab by 0530 that AM to be included.
Note: suspected sexual abuse cases will also require Chlamydia Transport Media.
Vaginal and Endocervical Swab Specimens:
Refer to collection package instructions
Male Urethral Specimens
1. The patient should not have urinated for at least 1 hour prior to sample collection.
2. Insert the specimen collection swab (blue shaft swab in the package with the green printing) 2 to 4 cm into the urethra.
3. Gently rotate the swab clockwise for 2 to 3 seconds in the urethra to ensure adequate sampling.
4. Withdraw the swab carefully.
5. Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube.
6. Carefully break the swab shaft at the scoreline; use care to avoid splashing of contents.
7. Re-cap the swab specimen transport tube tightly.
1. The patient should not have urinated for at least 1 hour prior to specimen collection.
2. Direct patient to provide a first-catch urine (approximately 20 to 30 mL of the initial urine stream) into a urine collection cup free of any preservatives.
3. Collection of larger volumes of urine may result in specimen dilution that may reduce test sensitivity. Female patients should not cleanse the labial area prior to providing the specimen.
4. Remove the cap and transfer 2 mL of urine into the urine specimen transport tube using the disposable pipette provided. The correct volume of urine has been added when the fluid level is between the black fill lines on the urine specimen transport tube label.
5. Re-cap the urine specimen transport tube tightly. This is now known as the processed urine specimen.
1. Swab specimens must be transported to the laboratory in the swab specimen transport medium and tube. Swab specimens must be transported to the laboratory at 2°C to 30°C.
2. Urine specimens can be transported to the laboratory at 2°C to 30°C in either the primary collection device (urine cup) or in the urine specimen transport tube. Urine specimens must be transferred into the APTIMA specimen transport tube within 24 hours of collection and before being assayed.
Nucleic Acid Amplification
Chlamydia Trachomatis; Not detected
Neisseria Gonorrhea; Not detected