Children's Mercy Kansas City
Name
Chlamydia and Gonorrhea PCR

CMH Lab Section
Molecular Infectious Diseases

Specimen Requirements
ER/Urgent Care/SCAN Clinic:
Collect vaginal, rectal, cervical, urethral, or penile sample with polyester swab in Universal Transport Medium. Urine collection: first-catch urine (no more and no less than 20-30 mls) in polycup.
Teen Clinic:
Collect vaginal and rectal sample with Gen-Probe vaginal swab collection kit (orange label tube). Urine collection: first-catch urine (no more and no less than 20-30 mls) in polycup.

Availability
Batched-Monday/Wednesday/Friday
Specimens must arrive in the Lab by 0530 that AM to be included.

Special Instructions
Note: suspected sexual abuse cases will also require Chlamydia Transport Media.

Specimen Collection:
Vaginal and Endocervical Swab Specimens:
Refer to collection package instructions

Male Urethral Specimens
1. The patient should not have urinated for at least 1 hour prior to sample collection.
2. Insert the specimen collection swab (blue shaft swab in the package with the green printing) 2 to 4 cm into the urethra.
3. Gently rotate the swab clockwise for 2 to 3 seconds in the urethra to ensure adequate sampling.
4. Withdraw the swab carefully.
5. Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube.
6. Carefully break the swab shaft at the scoreline; use care to avoid splashing of contents.
7. Re-cap the swab specimen transport tube tightly.

Urine Specimens
1. The patient should not have urinated for at least 1 hour pr

Processing Instructions
Specimen Transport
1. Swab specimens must be transported to the laboratory in the swab specimen transport medium and tube. Swab specimens must be transported to the laboratory at 2°C to 30°C.

2. Urine specimens can be transported to the laboratory at 2°C to 30°C in either the primary collection device (urine cup) or in the urine specimen transport tube. Urine specimens must be transferred into the APTIMA specimen transport tube within 24 hours of collection and before being assayed.

Methodology
Nucleic Acid Amplification

Ranges
Reference ranges:
Chlamydia Trachomatis;  Not detected
Neisseria Gonorrhea; Not detected

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