Children's Mercy Hospitals and Clinics
Name
Alpha Beta Double Negative T-Cell

Synonyms
ALPS

CMH Lab Section
Flow Cytometry

Specimen Requirements
Monday-Friday: 3 mL blood in EDTA lavender tube
After 1400 Friday & weekends: ACD yellow tube

Cell suspensions (lymph node or spleen aspirates) in RPMI media. Must specify source.

Availability
Routine

Turn Around Time
48-hours

Special Instructions
For optimal test interpretation an Extended Immune Deficiency Panel (EIDP) and CBC with differential should be ordered with this test or have been completed in the preceding 3-5 days.

Processing Instructions
Blood: whole blood room temperature
RPMI media: refrigerate

Methodology
Flow Cytometry. Antibody staining for cell surface markers CD45, CD3, CD4, CD8, TCR alpha beta & TCR gamma delta.

Ranges
See interperative report

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