Alpha Beta Double Negative T-Cell
CMH Lab Section
Monday-Friday: 3 mL blood in EDTA lavender tube
After 1400 Friday & weekends: ACD yellow tube
Cell suspensions (lymph node or spleen aspirates) in RPMI media. Must specify source.
Turn Around Time
For optimal test interpretation an Extended Immune Deficiency Panel (EIDP) and CBC with differential should be ordered with this test or have been completed in the preceding 3-5 days.
Blood: whole blood room temperature
RPMI media: refrigerate
Flow Cytometry. Antibody staining for cell surface markers CD45, CD3, CD4, CD8, TCR alpha beta & TCR gamma delta.
See interperative report