Children's Mercy Kansas City
Name
Antinuclear Antibody Reflex

Synonyms
ANA Reflex

CMH Lab Section
Immunology

Specimen Requirements
1.5 mL of blood in a red top w/yellow ring gel tube

Availability
Routine

Turn Around Time
ANA ELISA & IFA - batched testing performed Tuesdays and Thursdays
DsDNA batched on Thursdays;
AENA (Antibodies to Extractable Nuclear Antigen)is a Sendout

Special Instructions
If ANA ELISA is >20 then ANA IFA will be done
If ANA IFA Titer is ≥160 then reflex AENA and dsDNA

Methodology
ANA- ELISA, IFA
DsDNA- IFA
AENA- Sendout

Ranges
ANA ELISA
Negative ≤20
Equivocal 20-60
Positive ≥60

ANA IFA - negative
DsDNA- negative
AENA- see report

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