Single Copy (sc) Probes for FISH

Single copy probe design ensures hybridization to one genomic location
Up to 50x greater resolution than BAC-based probes
Eliminates dependency on proprietary probes and methods like Cot-1 DNA
Signal strength equivalent to BAC probes with 3- to 5-probe combinations
Custom probes can be rapidly designed and validated using any coding or non-coding sequence
Probes directly produced from genomic DNA
Significantly lower cost compared to other commercial probes or microarray technology

Unleash your genetic diagnostic potential with single copy (SC) probes for FISH applications. SC technology eliminates extra steps and cost involved with BAC probes, and circumvents the signal distortion introduced by using Cot-1 DNA that can lead to misinterpretation of results. SC’s high resolution and 1:1 DNA sequence affinity without repeats provides a direct and clear result. FISH with SC technology provides loci information of both rare and common genetic differences while avoiding the cost of proprietary microarray chips, equipment, consumables, staff training, and analytical support infrastructure.

scFISH probes can be designed with as few as 2 kb, providing a resolution level that identifies both rare and common abnormalities in a single assay. This improved breakpoint delineation avoids potential for cross-hybridization to other loci. The higher resolution enables precise and quick identification of locations of inversion, deletion, and translocation breakpoints.

scFISH probes use standard laboratory equipment. Specialized equipment is not required. A pre-validated probe library is available for many common diseases and infectious agents. For new probes, the probe development process is highly standardized and rigorously validated.

Testing Comparison Chart
Genetic Testing Features	sc FISH	G-Banding	BAC - FISH	Microarray	arrayCGH	QMH
Cost per test	< $100	< $100	~$250	~$600	~$500	<$5
Uses typical, standard lab equipment	Yes	Yes	Yes	No	No	Yes
Uses standard, non-proprietary consumables	Yes	Yes	No	No	No	Yes
Eliminates Cot-1 distortions	Yes	na	No	No	No	Yes
Has resolution < 2kb	Yes	No	No	Yes	Yes	Yes
Provides visual chromosomal location information	Yes	No	Yes	No	No	No
Can develop custom sequence probes in < 2 days	Yes	na	No	No	No	Yes

SC technology can be used to design single copy and low copy DNA probes which can be used in any genomic experiment, such as FISH, QMH, microarray, arrayCGH, PCR, Southern blotting, and more. In the comparison chart, note the benefits of FISH with SC technology vs. other genetic assays.

Tim Wurst, CEO Eriban Wurst, Inc. 3101 Broadway, Suite 630 Kansas City, Missouri 64111 USA Phone 1.816.753.5329		Technology developed and patented by: